We live in the anthropocene, in the era of fast technological achievements, which provide us with unprecedented opportunities in creating, maintaining and improving our lives. At the same time, with a modern approach to natural sciences and biotechnology, man has acquired the ability to change life, the conditions of life of all species or nature in general. Amphibians are a group of vertebrates that is a bioindicator of environmental change (in Croatia and the world). What contributes to the trend of the decrease in the number of individuals and species,  the amphibian decline are also the so-called black spots on the roads. Participants in this research study were 112 students from the Faculty of Teacher Education of the University of Zagreb (N = 112). During the academic year  of 2018/19 were first and second year students. The aim of the research was to compare their attitudes about the familiarity with the problems of amphibian rescue and conservation project on the roads. Three hypotheses have been put forward: H1 When migrating from land to water, but also after the end of mating, from water back to land, amphibians cross over the roads and a large number of them end up as casualties under the wheels of cars. H2 By building roads, people have at some places cut multi-century migratory routes of amphibians, creating a problem that is not unique to Croatia, but to the whole world as well. H3 In order to reduce the decline in the number of amphibians caused by road mortality, it would be necessary to map black points, educate and volunteer in amphibian rescue and conservation project operations. The results show insufficient student motivation and unsatisfactory activity. Students (and other members of society) should be encouraged to volunteering, education, biological and ecological awareness.