The discharge of untreated dyes containing wastewater into the water bodies is damaging the environment and creating toxicity to the aquatic life. The use of waste biomass for the removal of dyes containing industrial wastewater is an efficient, and inexpensive material. The Tobacco waste stalks were explored during the current study for the removal of an important textile dye methylene blue (MB) from the dye solution. The efficiency of the Tobacco stalks for MB removal was explored in raw form (RT) and active carbon form, i.e., activated with acetic acid (TAC-AA). The batch adsorption was carried out at variable time intervals, initial dye concentration, pH and temperature. The removal efficiency of the TAC-AA was increased from 60 to 91% (30 mg g-1 to 45 mg g-1) at 50 mg L-1 due to activation of RT. The surface area of the TAC-AA and RT was found to be 224 m2 g-1 and 0.504 m2 g-1, respectively. The MB adsorption on TAC-AA and RT was best described by Langmuir and Freundlich isotherm models, respectively, explaining the physisorption and chemisorption nature of the adsorption. The adsorption data followed the pseudo second order kinetic model.